This role is not open to remote applicants and would require relocation to Seattle, WA.Starting Salary Range: $75,000 - $88,000 annually
Exemption Status: Exempt
Location Expectations: Onsite
Reporting To: Manager II, Technical Operations
Role DescriptionA-Alpha Bio is seeking a Research Associate II to support our Technical Operations team's execution of our AlphaSeq assay. In this role, you'll execute DNA and yeast library construction, matings, next-generation sequencing, cell sorting, and flow cytometry to support interrogation of protein-protein interactions. Ideal candidates will have strong organizational skills, impeccable attention to detail, and an interest in executing screening assays at scale.
Key Responsibilities- Execute DNA library construction.
- Perform yeast transformation and matings, cell sorting, and flow cytometry.
- Execute DNA library preparation and sequencing workflows for Oxford Nanopore Technologies and Illumina platforms.
- Support the development and optimization of standard operating procedures for existing and new experimental workflows.
- Develop a foundational understanding of and executional expertise in Technical Operations methods.
- May contribute to project teams, execute project-related tasks, and/or train new Research Associates.
Qualifications & ExperienceRequired
- Bachelor's degree in a relevant field with 2+ years of relevant postbaccalaureate laboratory experience in the biotechnology industry; or an equivalent combination of education and experience.
- Proficiency in standard molecular biology techniques, including DNA extractions, quantitative PCR, Gibson assembly, and fragment analysis.
- Experience with multi-parametric flow cytometry.
- Experience in a high-throughput screening or manufacturing environment.
- Experience working within a LIMS/ELN.
- Strong work ethic, good organizational skills, team orientation, and exquisite attention to detail.
- Flexibility in day-to-day schedule and willingness to work outside of standard hours when necessary, including weekends.
Preferred
- Operator experience in a regulated environment (CLIA, cGXP, etc.).
- Hands-on experience with Illumina or ONT NGS platforms.
- Experience with cell sorting.
- Experience using yeast as a model organism.
- Experience with Benchling as a platform.
- Experience using lab automation.
