PhD in Biological Engineering, Chemical Engineering, Cell Biology, Molecular Biology, or related field with 0-2 years of industry experience; or MS with 3-4 years; or Bachelor's with 6-8 years of relevant experience.
Extensive experience in viral transductions, including T-cell transduction assays and flow cytometry.
Familiarity with downstream purification of Lentiviral vectors and viral genome engineering is highly desired.
Strong written and verbal communication skills.
Responsibilities
Perform lentiviral vector stable producer cell line development processes including transfection, pool generation, cloning, characterization, and cell banking.
Conduct assays to assess quantity, potency, and infectivity of lentiviral vector preparations.
Support innovation through the development of novel workflows and applications.
Assist in research projects to develop viral vector therapeutics with enhanced quality attributes.
Collaborate effectively with cross-functional teams in support of the LV CLD platform.
Contribute to process development efforts, including scale-up and characterization across various systems.
Design experiments, analyze data, troubleshoot, and maintain detailed records.
Benefits
Opportunity to work in a nimble, forward-thinking synthetic biology startup.
Engagement in cutting-edge research and development in biological engineering.
Collaboration with a diverse team of experts in the field.
Potential for professional growth and development in a rapidly evolving industry.
Full Job Description
We are seeking a Scientist to join our Lentiviral Vector Cell Line Development team in Boston, MA. The ideal candidate has a proven track record developing lentiviral vector (LV) packaging and stable producer cell lines in an industry setting. In this role, you will play a central part in engineering stable producer cell lines for LV therapeutics while driving upstream process optimization and scale-up. This is a unique opportunity to work at a nimble, forward-thinking synthetic biology startup and help build the future of biological engineering.
About the Role:
Perform Lentiviral vector stable producer cell line development processes including transfection, pool generation, cloning, characterization, and cell banking using state-of-the-art equipment.
Perform assays to assess quantity, potency and infectivity of lentiviral vector preparations.
Support innovation through development of novel workflows and applications.
Support research projects to develop viral vector therapeutics with enhanced quality attributes such as infectivity, potency and/or cell targeting.
Work effectively as part of a cross-functional team in support of the LV CLD platform and in collaboration with Synthetic Biology and Process Development teams.
Contribute to process development efforts, including scale-up and characterization across Ambr15, Ambr250, and benchtop systems, as well as LV analytical assay development.
Design experiments, analyze data, troubleshoot, and maintain detailed records.
Represent LV CLD on project teams, making decisions for cell line development activities and effectively communicating results, progress and plans.
About You:
You have a PhD in Biological Engineering, Chemical Engineering, Cell Biology, Molecular Biology, or a related field with 0-2 years of hands-on industry experience with Lentiviral vector production and cell line development in support of gene therapies; alternatively, a MS with 3-4 years or Bachelors with 6-8 years of this type of experience.
Extensive experience performing viral transductions, including T-cell transduction assay, flow cytometry, and quantitative gene expression analyses (ddPCR or qPCR).
Experience with downstream purification of Lentiviral vectors, viral genome engineering, and/or host cell genome engineering related to virus production or producer cell line technologies is highly desired.
Strong written and verbal communication skills required.
About Asimov
Asimov's Science Fiction is a science fiction magazine that was founded in 1977 by Isaac Asimov. The magazine publishes short stories, novellas, and novelettes, as well as book reviews and essays on science fiction. Asimov's Science Fiction is published by Dell Magazines, which is a subsidiary of Penguin Random House. The magazine is headquartered in New York City.
