Position summaryWe are seeking a Research Scientist Level II to provide scientific leadership and technical execution of in vivo projects in lung fibrosis and T cell immunity against influenza. The position will involve in vivo mouse experiments, including viral infection models, bleomycin administration, in vivo lung-function testing (Flexivent), tissue extraction and enzymatic processing, flow cytometric analysis, and bioinformatics analysis. This person will have substantial experience in Immunology, Cell and Molecular Biology or related field and will help train and support other lab members.
Responsibilities- Plan and perform mouse infection experiments with respiratory and other viral pathogens, bleomycin administration (o.p. route) under ABSL-2 conditions
- Administer substances via multiple routes, including oropharyngeal, intranasal, intravenous (tail vein), and intraperitoneal
- Perform mouse dissections, extraction of various mucosal tissues, BAL, lymph nodes, lung perfusion, and lung function testing using Flexivent instrument
- Carry out enzymatic and mechanical tissue digestion to generate single-cell suspensions
- Plan and execute single-cell RNA sequencing experiments
- Design, run, and analyze multiparameter flow cytometry experiments, including panel design, compensation, data analysis (e.g., FlowJo, Prism); FACS cell sorting
- Ensure compliance with IACUC protocols, biosafety requirements, and institutional policies
- Keep detailed, organized online laboratory records and maintain reagent and sample inventories
- Present data at lab meetings and contribute to manuscripts and grant preparation
- Train and support other lab members
Required Education and ExperienceBachelor's Degree in Immunology, Cell and Molecular Biology or related scientific discipline; may require a Master's Degree for certain lab specialties.
Minimum of two (2) years' experience in a related area/laboratory setting.
Required CredentialsN/A.
Preferred- Masters or PhD in Immunology, Cell and Molecular Biology or related scientific discipline.
- Proficiency in multiparameter flow cytometry, cell sorting, and data analysis
- mouse handling, and substance administration via various routes. Must be able to perform these tasks independently
- ability to focus during long in vivo mouse experiments, tissue digests, flow cytometry and complex experimental planning
- strong commitment to excellence, resilience, and good communication
- Design experiments in collaboration with PI
- Background in immunology, cell and molecular biology, or related field
- Experience with ABSL-2 and BSL-2 work
Additional experience in one or more of the following areas is desirable:- Transcriptomic and/or genomic data analysis using R, Python, or related tools
- Advanced immunofluorescence microscopy
- Computational image analysis using ImageJ/Fiji, Python, Imaris or related tools
Personal qualities- Strong work ethic and commitment to rigorous, reproducible science
- Exceptional attention to detail during all aspects of experimental work: design, execution, analysis, and record keeping
- Highly organized, able to manage multiple time-sensitive experiments at once
- Excellent and regular written and verbal communication with PI and other lab members
- Professional, respectful, and collaborative with colleagues at all levels
- maintain shared work calendar with lab members
Compensation Range$32.87 - $49.31 per hour
Salary InformationThis compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.
Disclaimer for Out of State ApplicantsThis compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors
Benefits InformationSeattle Children's offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits.
