We are looking for a highly motivated and collaborative scientist to join our Bioprocess Development team, focused on cell line development (CLD). The successful candidate will be joining a multidisciplinary team to generate commercially viable cell lines for novel and complex therapeutic molecules including bispecific antibodies, Fc fusion proteins, and enzyme fusions.
Key Responsibilities
- Support the development of Denali's mammalian biotherapeutic production platform to generate commercially viable cell lines.
- Execute cell line development activities including transfection, selection, single cell sorting, clone imaging, clone selection, and clone banking for pipeline projects.
- Design, execute, and interpret cell line development experiments in collaboration with other scientists in the group, including the use of JMP statistical software for DOE experiments and generating figures.
- Write technical and summary reports including cell line development reports which serve as source documents for regulatory filings, effectively communicate findings with cross-functional teams, and participate in scientific discussions with scientific teams.
- Maintain accurate and detailed laboratory notebook and relevant documentation. • Cross-functionally support cell culture process development through bioreactor production runs and DOE-based screening experiments as necessary.
- Evaluate, optimize, and implement characterization assays such qPCR, Western blots, and flow cytometry.
Requirements
- BA/BS or MS degree in Molecular Biology/Biochemistry/Cell Biology or related field • Technical proficiency in mammalian cell culture and upstream process development preferably in an industrial laboratory
- 2+ years of experience working in a laboratory environment
- Experience in CHO cell line development for biologics in a CMC setting preferred.
- Proficiency with protein characterization techniques such as Octet, SDS-PAGE, Caliper, and HPLC
- Demonstrated ability to organize, analyze, and interpret experimental data, specifically in JMP and Excel
- Ability to work effectively and collaboratively with other scientists in cross-functional project teams
- Excellent oral presentation and written communication skills
Salary Range: $106,000.00 to $138,000.00 . Compensation for the role will depend on a number of factors, including a candidate's qualifications, skills, competencies, and experience. Denali offers a competitive total rewards package, which includes a 401k, healthcare coverage, ESPP and a broad range of other benefits. Learn more at https://www.denalitherapeutics.com/careers
This compensation and benefits information is based on Denali's good faith estimate as of the date of publication and may be modified in the future.
