We are looking for a talented virologist or molecular biologist on the scientist level experienced with the engineering, production and purification of viral vectors, including lentivirus, adenovirus and AAV; and with a deep understanding of the underlying receptor biology of viral vector transduction of cells, ideally in non-model organisms. This is a hands-on role.Key Responsibilities:
- Perform cloning of large viral genomes, with a focus on capsid modification by either rational design or directed evolution
- Perform transfections on primary cells, immortalized cells, and animal tissues
- Design, manufacture, purify, characterize, validate and test a range of viral vectors, at small and large scale
- Design experiments, generate and analyze datasets allowing to adapt vector strategies to use in avian systems
- Assess efficiency of workflows through cellular assays and NGS sequencing approaches
- Extract DNA, RNA, protein from animal tissue, design and run PCR, qPCR, RT-PCR, ELISA assays to quantify viral transgene and transcription, protein
- Conduct experiments and work on research problems
- Maintain excellent electronic laboratory records
- Work on project milestones
- Attend lab meetings, prepare reports and presentations on the progress of projects
Requirements- PhD (or BA with 7+ years of experience/MS with 5+ years of experience) in biological sciences, bioengineering, molecular biology, genetics, or any science related field, or equivalent experience
- Experience with cloning large libraries or large viral genomes
- Experience with transfection and transduction of difficult target cells/tissues
- Experience producing, purifying, characterizing, validating and testing of a range of viral vectors including adenovirus, lentivirus, AAV
- Deep understanding of the cellular biology, receptors for viral entry and trafficking, mechanisms involved in viral transduction, and immunology
- Experience with ultracentrifugation and with resin and column based viral purification methods
- Experience in genome engineering, cloning, DNA amplification, PCR, NGS, transfections, and other molecular biology skills
- Strong aseptic techniques and GLP procedures
- Exceptional organizational skills with the ability to effectively manage time and priorities
- Strong written and oral communication skills, as well as creative problem-solving skills
- Curious, eager, and hardworking
Preferred qualifications: - Experience working with Avian viruses or AAVs or adapting viral vector strategies for use in avian systems
- Extensive experience with and knowledge of adenoviral vectors
- Experience with viral capsid engineering by rational design or directed evolution
- Experience with viral capsid library engineering
BenefitsCompensation: base salary is expected to be $120-150K depending on experience, with additional bonus compensation, equity compensation, and benefits including health insurance, dental insurance, vision insurance, and 401(k).
