5-7 years of hands-on experience with cryo-EM data processing platforms
Proficient in building and refining atomic models using tools like Phenix and Coot
Ability to derive technical and biological insights from cryo-EM datasets
Deep understanding of single-particle cryo-EM and its common challenges
Experience interpreting cryo-EM data at various resolutions
Strong communication skills for presenting technical work to diverse audiences
Background in biochemistry, biophysics, or cell biology
Responsibilities
Process single-particle cryo-EM datasets from raw data to validated reconstructions
Build and refine atomic models based on processed data
Adapt and troubleshoot established cryo-EM processing workflows
Utilize internal software tools and processing pipelines effectively
Interpret results considering sample quality and experimental limitations
Present findings to stakeholders and address technical inquiries
Collaborate with cross-functional teams to align on project goals
Benefits
Healthcare coverage
Annual incentive program
Retirement benefits
Broad range of additional benefits
Full Job Description
About the role:
We are seeking a Cryo-EM (Senior) Scientist to independently execute cryo-EM data processing and interpretation across multiple programs. This role is responsible for delivering high-quality structural insights end-to-end. The ideal candidate will have deep hands-on experience with cryo-EM data processing platforms, a strong practical understanding of single-particle cryo-EM, comfort operating within established internal software environments, and the ability to communicate results clearly in collaborative and client-facing settings.
Responsibilities:
Independently process single-particle cryo-EM datasets from raw movies through validated reconstructions
Build, refine and help interpret atomic models
Apply, adapt, and troubleshoot established processing workflows
Learn and efficiently use internally developed software tools and processing pipelines as a primary user
Interpret results in the context of sample quality, data collection conditions, and experimental limitations; and communicate implications clearly
Present results to internal stakeholders and external clients, fielding technical questions and defending conclusions in real time
Collaborate with experimental, computational, and software teams to align on collective needs and directions across programs
Qualifications
Demonstrated ability to independently process cryo-EM data using established tools (RELION, cryoSPARC, cisTEM, etc.)
Demonstrated experience building and refining atomic models in Phenix, Coot, ChimeraX/Isolde, etc.
Demonstrated ability to draw relevant technical and biological insights from processed datasets, and use those insights to suggest next steps
Deep practical understanding of single-particle cryo-EM, including common failure modes and tradeoffs related to sample quality (e.g., heterogeneity, preferred orientation, ice quality, particle behavior) and how these factors affect processing strategies and results
Experience using insights from cryo-EM data at multiple resolutions: complex formation, protein-protein interaction mapping, high-resolution, etc.
Demonstrated experience presenting technical or scientific work and the ability to clearly articulate results, assumptions, and technical tradeoffs in discussions with internal and external stakeholders
Strong background in biochemistry, biophysics, and/or cell biology; and an ability to communicate and collaborate with protein scientists to facilitate project success.
Comfort onboarding into and operating within existing software tools and workflows without owning their development
Experience working with diverse projects and samples, and operating in a dynamic environment
Creativity and excitement to advance the frontiers of capability, throughput, and speed in structural biology.
Additional Strengths (at least one preferred)
Experience with scripting (python, shell) in support of cryo-EM data analysis
Strong working knowledge of UNIX/Linux environments and large-scale data handling
Cryo-EM grid preparation and vitrification
Cryo-EM microscope operation or data acquisition
Deep understanding of complementary structure determination methods such as X-ray crystallography or NMR spectroscopy
Experience with cryo-ET, milling, and tomographic reconstruction methods
The salary range for this role is $115,000 - $184,300. Compensation for the role will depend on a number of factors, including a candidate's qualifications, skills, competencies, and experience. Fathem currently offers healthcare coverage, annual incentive program, retirement benefits and a broad range of other benefits. Compensation and benefits information is based on Fathem's good faith estimate as of the date of publication and may be modified in the future.
About Flagship Pioneering
Flagship Pioneering is a life sciences innovation enterprise that conceives, creates, resources, and develops first-in-category life sciences companies. The firm operates through its Flagship Labs unit, which is a dedicated innovation foundry that invents and launches transformative companies. Flagship Pioneering has created over 100 scientific ventures, resulting in over $34 billion in aggregate value, 500+ issued patents, and more than 50 clinical trials for novel therapeutic agents. The firm's portfolio includes companies in the fields of therapeutics, health technologies, and sustainability. Flagship Pioneering was founded in 2000 and is headquartered in Cambridge, Massachusetts.
