The RoleProfluent is scaling the discovery engines that supply data for AI-driven protein design. We're hiring a Scientist II to build and own our yeast surface display platform for binder generation. The data you produce will train and evaluate our protein design models, and the binders that emerge will open up new directions for the company.
This role sits within the Molecular Function (MF) program, our cross-functional effort spanning ML, Biology, and Bioinformatics. You will be the scientific owner of the yeast display platform: building it hands-on, from library construction through FACS and NGS readout, then setting its direction as it scales with Profluent's design ambitions.
As the platform matures, this role will grow towards setting direction for how display and protein engineering evolve at Profluent, including how we build on the most interesting binders the platform uncovers. We are looking for a yeast display expert who is ready to take on that broader scope.
Responsibilities- Establish and validate a yeast surface display platform for binder generation, including display vector and strain selection, library design and construction, and FACS-based selection and sorting
- Design and execute yeast display campaigns across binder formats (e.g., de novo miniproteins, VHHs, scFvs, and alternative scaffolds) against a range of targets
- Take workflows from concept to a robust, repeatable process: define controls, QC, and acceptance criteria, and characterize reproducibility, signal-to-noise, and the relationship between sort-bin enrichment and binding strength
- Build NGS readouts into selections and deep mutational scanning workflows, converting raw sequencing counts into quantitative binding metrics that ML models can learn from
- Partner with the ML scientists, and Protein Design scientists to scope campaigns (targets, library composition, readouts) and to define what "model-ready" binding data looks like
- Work with Bioinformatics on metadata schemas and data ingestion so every dataset is traceable to its parent campaign
Qualifications- PhD in Molecular Biology, Biochemistry, Protein Engineering, Biophysics, Immunology, or a closely related field, with 3+ years of relevant industry experience (level and compensation will be commensurate with experience)
- Deep hands-on experience with yeast surface display, including library construction (e.g., homologous recombination, electroporation at scale), induction and expression optimization, and multi-round selection campaigns (required)
- Proven track record of generating and screening libraries for binder discovery or optimization (naive, immune, synthetic, or designed)
- Strong flow cytometry and FACS expertise, including gating strategy, sort design, and data interpretation
- Working knowledge of NGS-based screening workflows (e.g., display library sequencing, deep mutational scanning, amplicon sequencing) and how to turn that data into a quantitative readout
- Strong molecular biology skills (cloning, mutagenesis, DNA assembly)
- Demonstrated ability to define and uphold assay quality standards: controls, reproducibility, and signal-to-noise
- Solid understanding of protein engineering and developability considerations
- Comfortable working across disciplines: can discuss binding biophysics with biologists and model-guided design with ML scientists
Preferences- Experience with titration-based affinity measurement by display and sequencing (Tite-Seq-style) or analogous approaches to recovering KD from pooled data
- Experience with display-based affinity maturation and binder engineering
- Experience with antibody characterization (e.g., Carterra SPR, Octet BLI) and protein expression/purification
- Experience designing experiments specifically to generate ML training data (e.g., focused libraries, systematic mutagenesis, library-on-library screens)
- Experience with additional display platforms (phage, mammalian, ribosome/mRNA)
- Experience with lab automation (e.g., Hamilton, Tecan) and LIMS/ELN systems (e.g., Benchling)
- Experience establishing a new platform and transitioning it to routine execution, including training others to run it
- Prior startup experience or comfort in fast-paced, ambiguous environments
What We Offer - High-growth opportunity with meaningful impact on the future of protein design
- Competitive compensation package with equity participation
- 401(k) with a strong employer match
- Comprehensive benefits including health/dental/vision insurance
- Generous PTO policy and commitment to work-life balance
- Professional development opportunities in a cutting-edge field at the intersection of AI and biology
Hiring Salary Range$155,000-$160,000 USD
NOTICE TO STAFFING AND SEARCH FIRMS: Profluent does not accept unsolicited candidate submissions. We ask that agencies refrain from sending resumes to our careers inbox, hiring managers, or any Profluent employee. Any resume submitted without a signed agreement and an active search request from our Talent team will be treated as the property of Profluent, and no placement fee will be owed.
