We are seeking a Scientist level molecular biologist to lead DNA construct design, high-throughput cloning, and sequence quality control for our AI-driven antibody and protein discovery platform. This hands-on role will deliver sequence-verified constructs to support a range of research and expression applications, while maintaining accurate, traceable records linking each molecular design to its corresponding physical sample.
This is full-time onsite in Bothell, WA.
Responsibilities- Design expression constructs and primers, selecting appropriate vectors, regulatory elements, tags, and codon usage for the intended expression system.
- Execute and troubleshoot cloning, assembly, and site-directed mutagenesis using methods such as Gibson assembly, Golden Gate, restriction-ligation, and In-Fusion.
- Develop and scale plate-based cloning workflows, including bacterial transformation, colony screening, plasmid preparation, and sequence verification.
- Review Sanger and whole-plasmid sequencing data against intended designs; investigate sequence discrepancies and document construct release decisions.
- Maintain annotated, version-controlled plasmid maps and sequence records in Geneious with traceability to sample IDs and storage locations.
- Establish naming conventions, annotation standards, QC acceptance criteria, and SOPs; monitor cloning success and turnaround time.
- Partner with protein science and automation teams to transfer verified constructs into expression workflows and improve reproducibility and throughput.
Qualifications - PhD in molecular biology, biochemistry, or a related field with 0-3 years of relevant industry experience.
- Strong practical expertise in DNA construct and primer design, molecular cloning, and troubleshooting, including proficiency in at least two assembly or cloning approaches.
- Proficiency in Benchling, Geneious, or comparable software for sequence analysis, alignment, plasmid annotation, and construct record management.
- Experience interpreting sequencing results, identifying sequence errors, and maintaining accurate plasmid maps and sample records.
- Experience with parallel or plate-based cloning workflows, strong attention to detail, and clear documentation and cross-functional communication.
Preferred Qualifications- Experience with 96-well cloning, whole-plasmid NGS analysis, or liquid-handling automation.
- Antibody construct design across formats such as Fab, IgG, VHH, scFv, and multispecific proteins.
- Python or other scripting skills for sequence processing, QC, or workflow automation.
- Familiarity with protein purification and characterization, including ÄKTA, SEC, BLI, or SPR.
Compensation and Benefits:
The expected base salary range for this position is $90,000 - $115,000 annually. Actual compensation will be based on a variety of factors, including but not limited to a candidate's level qualifications, experience, and skills.
While most offers typically fall within the low to mid-point of the range, we may extend an offer toward the higher end for exceptional candidates whose background and expertise exceeds the requirements of the role.
SystImmune offers a comprehensive benefits package including: 100% paid employee premiums for medical/dental/vision, also STD, LTD, a 401(k) plan with a 50% company match of up to 3% and a vesting schedule of only 5 years, 15 PTO days per year, sick leave, plus 11 paid holidays and MORE.
