Location Memphis, TN
Category Laboratory Sciences & Support
Department Structural Biology
Shift Weekday Day
Position Type Full Time
Scheduled Weekly Hours 40
Req # JR7952
Job DescriptionWe are looking for a Lead Researcher to participate in our exciting studies of the human protein kinome.
This
Lead Researcher position is part of the Blue Sky Initiative "
Seeing the Invisible in Protein Kinases" at
St. Jude Children's Research Hospital in Memphis, TN. The Blue Sky Initiative "Seeing the Invisible in Protein Kinases" is an inter-disciplinary project in
the Department of Structural Biology and is supported by US $50 million in institutional funding. This large-scale project leverages ultra-high field nuclear magnetic resonance (NMR), complemented with cryoEM, X-ray crystallography, data-science and computational approaches, to characterize the conformational landscape of the human kinome.
This knowledge will then be used to discover new biology of kinase function and regulation in cells, understand the structural and functional consequences of disease-associated mutations, and identify novel therapeutic opportunities. As a member of the Protein Technology Centre /BlueSky team, the candidate will use molecular biology, biochemistry, biophysical, and structural biology skills to study the molecular mechanisms and structures of protein kinases and their cellular regulatory functions.
As a member of the
Protein Technology Centre/BlueSky team, the candidate will use their molecular biology, biochemistry, and biophysical skills to study the molecular mechanisms and structures of protein kinases and their cellular regulatory functions. Additionally, the candidate will assist in preparing high-quality proteins from various expression systems such as bacteria, insect cells and mammalian cells for structural studies of kinases using NMR, crystallography and cryo EM. The work is diverse, providing an opportunity to study multiple kinases, perform appropriate background research, optimize biochemical and biophysical assays and structural characterizations. Not only do the biophysical and structural studies characterize the molecular mechanisms of protein kinases, but they are also used to discover new mechanisms of kinase regulation and/or new kinase-mediated biology.
Job Responsibilities:- Independently perform and lead molecular cloning and protein purification experiments
- Biophysically characterize the proteins and perform various biochemical assays
- Screen protein crystals for X-ray crystallography and freeze grids for Cryo-EM
- Handle insect and mammalian cell cultures for protein production
- Experience with at least one structural biology technique, such as cryo-EM or X-ray crystallography, and the ability to refine, model-build, and interpret protein structures
- Analyze and interpret experimental data and prioritize experiments for structural studies
- Maintain comprehensive and well-organized experimental records
Minimum Education and/or Training:- Bachelor's degree in a relevant scientific area is required
Minimum Experience:- 5+ years of research experience is required with a Bachelor's degree.
- 3+ years of experience is required with a Master's degree.
- No prior experience required with a PhD.
- Strong knowledge and experience in understanding and application of underlying principles related to techniques being performed.
- Proven performance in earlier role/comparable role.
CompensationIn recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $60,320 - $105,040 per year for the role of Lead Researcher - Protein Technologies Center - Blue Sky Kinases .
Explore our exceptional benefits!
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