Scientist as part of the
Research and Development team based in
Philadelphia, PA. Role Overview Legend Biotech seeks an experienced Scientist to join the Upstream team, Vector Biology in Philadelphia, with a focus on in-vivo CAR-T programs. This independent contributor role will own hands-on execution and data-driven
development of lentiviral vector upstream processes, including seed train strategy and scale-up from small-scale to 40-Liter bioreactor runs. This Scientist position will closely work with Molecular Biology, Downstream, Analytical and Technical development partners to ensure end-to-end process alignment, support comparability strategy and readiness, and enable efficient, high-quality technology transfer.
Key Responsibilities- Define and optimize the upstream process from shake flask through bioreactors, scaling to 40-L with reproducible performance and clear rational for operating parameters.
- Execute and optimize lentiviral vector production workflows supporting in-vivo CAR-T application, including transient transfection optimization and/or stable cell line optimization.
- Perform media optimization, feeding strategy development, and process control adjustments to improve yield, quality, scalability and robustness.
- Develop and execute fit-for-purpose studies to support scalability and readiness.
- Collaborate with internal teams within Vector Biology and coordinate cross-functional activities to support technology transfer to internal Technical Development team.
- Contribute technical input to cross-functional discussions on experimental design, comparability strategy, and progression of exploratory work into formal process development.
- Independently plan, perform, analyze, and interpret experiments with minimal supervision.
- Deliver clear data summaries and evidence-based recommendations to Vector Biology and cross-functional teams.
- Prepare high-quality technical reports, study summaries, and slide decks to support decisions on the upstream process development.
- Adhere to BSL-2 biosafety standards and Legend policies, maintaining accurate and compliant electronic lab notebooks and documentation.
- Ensure experimental reproducibility and data integrity through detailed and organized record-keeping.
Requirements - MS degree with 4+ years; or PhD degree with 1 year's prior industry experience in Bioengineering, Virology, Cell biology, Chemical Engineering, or related field.
- Hands-on experience in viral vector production, and characterization.
- Experience with mammalian suspension cell culture, transient transfection, and upstream bioprocess development.
- Strong technical background in molecular biology techniques, including plasmid construction, PCR/qPCR/dPCR based assay, and cell line development.
- Demonstrated ability to design, execute, analyze, and troubleshoot complex experiments in a fast-paced R&D environment.
- Experience scaling upstream processes from shake flasks to bioreactor system (up to 50 L preferred).
- Experience supporting gene therapy platform development, preferably involving lentiviral vector applications.
- Basic understanding of upstream process development and their impact on product quality.
- Strong organizational and time management skills, with the ability to manage multiple projects and priorities.
- Excellent analytical and problem-solving skills with the ability to interpret data and recommend next steps.
- Proficiency in maintaining accurate experimental records and documenting data using electronic laboratory notebooks (ELNs) to ensure data traceability.
- Excellent verbal and written communication skills, with experience preparing technical reports and presenting scientific findings to cross-functional teams.
- Ability to work both independently and collaboratively in a multidisciplinary research environment.
- Adaptable, self-motivated, and eager to learn new technologies and experimental approaches.
#Li-JR1
#Li-Onsite
The base pay range below is what Legend Biotech USA Inc. reasonably expects to offer at the time of posting. Actual compensation may vary based on experience, skills, qualifications, and geographic location. The company reserves the right to modify this range as needed and in accordance with applicable laws.
Other Types of Pay: Performance-based bonus and/or equity is available to employees in eligible roles.
Benefits and Paid Time Off: Medical, dental, and vision insurance as well as a 401(k) retirement plan with a company match that vests fully on day one. We offer eight (8) weeks of paid parental leave after just three (3) months of employment, and a paid time off policy that includes vacation time, personal time, sick time, floating holidays, and eleven (11) company holidays. Additional voluntary benefits include flexible spending and health savings accounts, life and AD&D insurance, short- and long-term disability coverage, legal assistance, and supplemental plans such as pet, critical illness, accident, and hospital indemnity insurance. We also provide voluntary commuter benefits, family planning and care resources, well-being initiatives, and peer-to-peer recognition programs; demonstrating our ongoing commitment to building a culture where our people feel empowered, supported, and inspired to do their best work.
Pay Range (Base Pay):
$96,267-$126,351 USD
Please note: These benefits are offered exclusively to permanent full-time employees. Contractors are not eligible for benefits through Legend Biotech. 
