THE ROLEWe are seeking a highly motivated Senior Research Associate to support the development and optimization of microbial fermentation processes for recombinant protein production. Reporting to the Principal Scientist, Bioprocess Development, this individual will play a key role in executing and scaling aerobic microbial fermentation workflows in a fast-paced startup environment.
The ideal candidate is highly hands-on, technically rigorous, and comfortable operating independently in a dynamic research setting. This role requires strong practical experience with bench-scale bioreactors, microbial culture techniques, fermentation analytics, and laboratory operations.
KEY RESPONSIBILITIESFermentation Operations- Operate, monitor, and troubleshoot bench-scale aerobic microbial fermentation systems with minimal supervision
- Execute batch and fed-batch fermentation experiments to support process development objectives
- Prepare media, inoculum, feeds, and process solutions for fermentation campaigns
- Perform reactor setup, sterilization, calibration, and post-run cleaning/maintenance
Sampling & Analytical Support- Collect and process fermentation samples throughout cultivation runs
- Perform routine analytical assays including OD measurements, metabolite analysis, and sample preparation for HPLC or related analytical platforms
- Track fermentation performance metrics including biomass, substrate consumption, and product formation
Strain Evaluation & Process Development- Support screening and characterization of microbial strains in shake flask and bench scale bioreactors
- Assist in process optimization studies, including media development and fermentation parameter evaluation
- Maintain accurate and well-organized experimental records and documentation
Laboratory Operations- Generate and maintain microbial cell banks and inventory records
- Coordinate ordering and tracking of fermentation consumables and raw materials
- Support routine laboratory maintenance, equipment upkeep, and troubleshooting
- Contribute to safe laboratory operations and maintain a strong culture of safety and operational rigor
Cross-Functional Collaboration- Collaborate with strain engineering, downstream, and analytical teams to support integrated process development activities
- Assist with scaling workflows and implementing improved operational practices as the platform grows
PROFESSIONAL EXPERIENCE & QUALIFICATIONS - Bachelor's degree (with 5-7 years of relevant research experience) or Master's degree (with 3-5 years of relevant research experience) in Bioengineering, Chemical Engineering, Microbiology, Biotechnology, or related discipline.
- Hands-on experience in microbial fermentation, microbiology, bioprocess development, or related laboratory environments
- Experience in operating and maintaining bench-scale bioreactors
- Strong understanding of aseptic technique and microbial culture handling
- Experience with aerobic microbial fermentation workflows
- Strong organizational and documentation skills
- Ability to independently execute experiments and troubleshoot routine process issues
Preferred Experience- Experience with fed-batch fermentation processes
- Familiarity with HPLC-based analysis of sugars, organic acids, or fermentation metabolites
- Experience with recombinant protein expression systems
- Experience supporting process optimization or scale-up activities
- Familiarity with fermentation data analysis and process monitoring tools
LOCATION: Cambridge, MA
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The salary range for this role is $81,000 - $107,300. Compensation for the role will depend on a number of factors, including a candidate's qualifications, skills, competencies, and experience. Protocos currently offers healthcare coverage, annual incentive program, retirement benefits and a broad range of other benefits. Compensation and benefits information is based on Protocos's good faith estimate as of the date of publication and may be modified in the future.
