Site: The McLean Hospital Corporation

Job Summary
McLean Hospital is a comprehensive psychiatric hospital committed to providing easy access to superior quality, cost-effective mental health services in the Boston area, Massachusetts and beyond. Since 1811, McLean Hospital has been a world leader in the treatment of mental illness and chemical dependency, research into the cause of mental illness and the training of generations of mental health care providers. McLean's multidisciplinary programs treat a broad range of psychiatric illnesses across the full continuum of care.

All McLean team members are expected to consistently demonstrate our values of integrity, compassion, respect, diversity, teamwork, excellence and innovation in their work activities and interactions.

A post-doctoral fellow typically holds dual affiliations at McLean Hospital and Harvard Medical School.

There are many opportunities for education and growth in our multidisciplinary, collaborative research environment at McLean Hospital that brings together a diverse group of neurobiologists, clinicians, psychologists and statisticians. Please see https://www.mcleanhospital.org/research
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Job Description
With supervision and guidance, responsibilities of the Postdoctoral Research Fellow will include:
oPerforms independent and highly specialized experimental procedures
oDesigns and implements modifications of research protocols
oTroubleshooting problems and instructing colleagues in highly specialized techniques
oSynthesizes and interprets results
oPerforms advanced data recording and analysis using various software programs and statistical techniques
oMaintaining organized and detailed records of laboratory experiments in electronic laboratory notebooks
oPresentation of results at lab meetings and national conferences
oPreparation and submission of research manuscripts to peer-reviewed journals
oAssisting with preparation of grant applications
oOther duties as assigned

A successful candidate will:
oWork independently under general direction of the Principal Investigator
oBe motivated, meticulous, organized, and detail-oriented
oDemonstrate initiative and enthusiasm for learning new techniques
oHave strong analytical and problem-solving skills
oPossess strong technical abilities and computer science skills
oPossess exceptional written and oral communication skills as demonstrated by publications and presentation
oPossess the ability to make independent and effective decisions in a timely fashion
oPossess competence in research techniques and methodologies
oHave excellent interpersonal skills
oBe a team player who works well with other lab members

Additional Job Description

Postdoctoral Fellow in Translational Neuroscience and McLean Hospital Microscopy Core Manager
The Translational Neuroscience Laboratory at McLean Hospital and Harvard Medical School seeks a highly motivated scientist for a combined position as a postdoctoral fellow and Manager of the McLean Hospital Microscopy Core. This position offers a distinctive opportunity to pursue research on the cellular and molecular mechanisms underlying psychiatric disorders while providing scientific and operational leadership for a shared advanced-microscopy facility.
Postdoctoral Research Program
The postdoctoral fellow will contribute to a multidisciplinary research program examining how stress and immune signaling affect interactions among neurons, glia, and the neurovascular unit (NVU). The NVU-including brain endothelial cells, pericytes, astrocytes, microglia, neurons, and the vascular basal lamina-is positioned at the interface between systemic and neural processes and may play a critical role in translating immune, metabolic, and other stress-related challenges into altered neural function and psychiatric symptoms.
The research investigates the hypothesis that stress-related symptom dimensions-including anxiety, irritability, depression, and anhedonia-are associated with dysregulation of NVU homeostatic reserve: the capacity of NVU cells to respond adaptively to stress and immune challenges. Studies will examine coordinated gene-expression programs across neuronal, glial, and vascular cell populations and determine how their disruption relates to clinically relevant symptom dimensions across psychiatric diagnoses.
The fellow will work with deeply characterized human postmortem brain specimens, primarily from the amygdala and anterior cingulate cortex, and with cellular preparations derived from the same donors. Molecular, cellular, and morphological findings will be integrated with transdiagnostic clinical phenotypes derived from extensive health records using natural-language-processing approaches.
Research activities may include:
• Advanced fluorescence, confocal, two-photon, and electron microscopy.
• Quantitative image analysis and three-dimensional reconstruction.
• Multiplex in situ RNA and protein detection in human brain tissue.
• Morphological and cytoarchitectonic analyses of neurons, glia, microvasculature, and other NVU components.
• Primary human astrocyte, microglial, and brain endothelial cell cultures derived from postmortem tissue.
• iPSC-derived glial and vascular cell models, including comparisons with primary cells and native tissue from the same donors.
• Experiments examining cellular responses to cytokines, stress neuropeptides, and immune or metabolic challenges.
• Single-cell or single-nucleus transcriptomic studies of brain tissue, isolated microvasculature, and cultured cells.
• Integration of molecular, cellular, morphological, and functional measures with transdiagnostic dimensions of stress-related symptoms.
The fellow will be encouraged to develop independent research questions within this program and will contribute to experimental design, data analysis and interpretation, scientific presentations, manuscript preparation, and grant applications.
Microscopy Core Management
In parallel with the postdoctoral research program, the successful candidate will manage the scientific, technical, operational, and financial activities of the McLean Hospital Microscopy Core. The Core Manager will serve as a front-line scientific resource for McLean investigators and external users, providing expert guidance on microscopy-based study design, sample preparation, data acquisition, image processing, and quantitative analysis.
The Core Manager will oversee the overall functioning of the facility, coordinate instrument access and maintenance, train and support users, supervise contracted technical services, and serve as a liaison among investigators, hospital administrative units, vendors, and external service providers. The role also includes consultation on microscopy-related methods, project feasibility, budgeting, and appropriate use of Core resources.
Transmission Electron Microscopy
Responsibilities will include:
• Maintain the Reichert-Jung ultramicrotome and ancillary TEM equipment.
• Oversee the operation and maintenance of the JEOL JEM1200EX transmission electron microscope and associated cooling systems.
• Conduct TEM studies for McLean and external investigators.
• Provide expertise in resin embedding, sample preparation, ultramicrotomy, staining, image acquisition, and image processing.
• Perform and support immuno-electron microscopy.
• Perform cryopreservation and freeze substitution of biological specimens.
• Conduct electron tomography on ultrathin sections and reconstruct tomographic datasets.
• Perform negative staining for appropriate biological samples.
• Prepare specialized sample supports, including Formvar- and carbon-coated grids, glow-discharged grids, and grids containing gold fiducial markers.
• Advise investigators on experimental design, sample suitability, controls, and interpretation of ultrastructural findings.
• Coordinate and supervise on-site contracted TEM maintenance and repair services.
Laser-Scanning Confocal Microscopy
Responsibilities will include:
• Train users in independent operation of the Leica SP8-TCS laser-scanning confocal microscope.
• Train and support users in image acquisition, processing, visualization, and quantitative analysis.
• Provide consultation on fluorophore selection, spectral separation, imaging parameters, controls, resolution, sampling, and avoidance of imaging artifacts.
• Perform confocal microscopy for McLean and external investigators when specialized assistance is required.
• Maintain the confocal microscope and associated hardware and software in optimal operating condition.
• Diagnose technical problems and coordinate preventive maintenance, repairs, and software or hardware upgrades.
• Supervise on-site contracted services related to the confocal system.
Two-Photon Laser-Scanning Microscopy
Responsibilities will include:
• Train users to operate the Olympus FV-MPE-RS two-photon microscope and Spectra-Physics InSight DeepSee Duo laser system.
• Provide guidance on experimental design, image acquisition, image processing, and quantitative analysis for two-photon applications.
• Support investigators in optimizing imaging parameters for fixed tissue, living preparations, and other appropriate applications.
• Maintain the microscope, laser system, environmental components, and associated software in optimal working condition.
• Coordinate and supervise preventive maintenance, repair, and other contracted services related to the two-photon system.
Core Operations and Research Administration
The Core Manager will:
• Develop and maintain a structured, equitable user registration and instrument-scheduling system.
• Establish and maintain instrument-specific training, authorization, and competency records.
• Develop, update, and enforce standard operating procedures for instrument use, sample preparation, data management, and laboratory safety.
• Monitor instrument usage, performance, service history, and user needs.
• Ensure compliance with laboratory safety, security, biosafety, chemical handling, and hazardous-waste-disposal requirements.
• Maintain adequate inventories of laboratory consumables, reagents, replacement parts, and essential supplies.
• Coordinate relationships with vendors, service engineers, contractors, and institutional administrative units.
• Assist investigators with microscopy-related study design, feasibility assessment, cost estimates, and budget preparation.
• Maintain accurate records of Core projects, users, instrument time, services, and technical support.
• Contribute to strategic planning for equipment replacement, upgrades, and acquisition of new imaging technologies.
• Promote effective and rigorous use of microscopy resources across the McLean research community.
Fiscal Management
Responsibilities will include:
• Generate and issue monthly invoices for instrument usage and Core services.
• Develop and manage the annual operating budget for the Microscopy Core.
• Track service contracts, maintenance expenses, consumables, user charges, and other Core expenditures.
• Maintain detailed and accurate financial records.
• Assist in establishing and periodically reviewing instrument and service rates.
• Provide usage and financial reports to departmental and institutional leadership as requested.
• Contribute technical information and budget estimates for grants involving Core instrumentation or services.

Qualifications

Qualifications

Candidates should hold a PhD, MD, or equivalent doctoral degree in neuroscience, cell biology, molecular biology, biomedical imaging, neuropathology, or a related discipline. The position requires substantial hands-on experience with advanced microscopy and the scientific judgment needed to guide investigators in experimental design, image acquisition, analysis, and interpretation.

Preferred qualifications include:

• Demonstrated expertise in transmission electron microscopy and/or advanced fluorescence microscopy.
• Experience with biological sample preparation, ultramicrotomy, immuno-electron microscopy, confocal microscopy, two-photon microscopy, or quantitative image analysis.
• Knowledge of microscopy instrumentation, troubleshooting, preventive maintenance, and quality control.
• Experience training and supporting users with different levels of microscopy expertise.
• Experience with human postmortem tissue, neuroanatomy, neuropathology, cell culture, neuroimmunology, blood-brain barrier biology, or transcriptomics.
• Strong organizational, administrative, and record-keeping skills.
• Experience with budgeting, billing, shared-resource management, or research administration.
• Excellent interpersonal and communication skills and the ability to work effectively with investigators, trainees, administrators, vendors, and external users.
• A strong commitment to experimental rigor, reproducibility, collaborative science, and responsible stewardship of shared research resources.

Training and Research Environment

The successful candidate will join a highly collaborative translational neuroscience environment at McLean Hospital and Harvard Medical School, with access to exceptional human brain resources, advanced imaging and genomic technologies, and multidisciplinary expertise. The combined position provides opportunities to develop an independent scientific program, acquire complementary experimental and computational skills, build expertise in research-core leadership, publish findings, present at national and international conferences, and pursue fellowship and career-development funding.

Qualifications

Candidates should hold a PhD, MD, or equivalent doctoral degree in neuroscience, cell biology, molecular biology, biomedical imaging, neuropathology, or a related discipline. The position requires substantial hands-on experience with adva