We are looking for a talented molecular biologist on the RA, SRA or scientist level experienced with the engineering, production and purification of viral vectors, including lentivirus, adenovirus and AAV.Key Responsibilities:
- Perform cloning of large viral genomes, design and run PCR, qPCR, RT-PCR assays to quantify viral transgene and transcription
- Perform transfections on primary cells, immortalized cells, and animal tissues
- Design, manufacture, purify, characterize, validate and test a range of viral vectors, at small and large scale
- Assess efficiency of workflows through cellular assays and NGS sequencing approaches
- Extract DNA, RNA, protein from animal tissue
- Conduct experiments and work on research problems
- Maintain excellent electronic laboratory records
- Work on project milestones
- Attend lab meetings, prepare reports and presentations on the progress of projects
Requirements- BA/MS/PhD in biological sciences, bioengineering, molecular biology, genetics, or any science related field, or equivalent experience
- Experience with cloning, transfecting, producing, purifying, characterizing, validating and testing of a range of viral vectors including adenovirus, lentivirus, AAV
- Experience with ultracentrifugation for viral purification
- Experience with resin and column based viral purification methods
- Strong aseptic techniques and GLP procedures
- Experience in genome engineering, cloning, DNA amplification, PCR, NGS, transfections, and other molecular biology skills
- Exceptional organizational skills with the ability to effectively manage time and priorities
- Strong written and oral communication skills, as well as creative problem-solving skills
- Curious, eager, and hardworking
Preferred qualifications: - Experience with viral capsid engineering by rational design or directed evolution
- Extensive experience with and knowledge of adenoviral vectors
- Experience with viral capsid library engineering
BenefitsCompensation: base salary is expected to be $90-130K depending on experience, with additional bonus compensation, equity compensation, and benefits including health insurance, dental insurance, vision insurance, and 401(k).
