Please note that this position is contingent upon the successful award of a contract currently under bid. Summary: The Senior Bacteriology Technician shall assist the Lead/PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence. Assist in approximately 2 animal protocols conducted by other investigators. Responsibilities Essential Job Functions: • Shall prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc). • Contractor shall plate approximately 100 LB plates for use for Ames sporulation isolation and purification. • Contractor shall isolate and purify approximately 10 plasmid DNA specimens. • FITC label and count approximately 10 Ames samples to check ratio of Bacilli: Spores over multiple time points. • Contractor shall order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment - approximately 400 items ordered along with 5 pieces of equipment. • Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator. Use computer to prepare graphs and write reports for supervising investigator. • Contractor shall perform approximately 100 Immunological, antibody determinations. • Contractor shall perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum. • Contractor shall perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum. Measures cytokines in cell cultures and cell extracts. Uses a Luminex instrument for analysis. • Contractor shall perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression. Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution. • Perform other duties as necessary. Qualifications Necessary Skills and Knowledge: • Expertise in laboratory safety and aseptic techniques. • Proficiency in biological assay procedures (ELISA, PCR). • Ability to manage sample tracking and recordkeeping. • Possesses experience, skill and expertise with a large variety of the field's concepts, practices, and procedures. Minimum Qualifications: • Bachelor's degree in a related field. • Minimum 7 years of related experience. • Ability to obtain and maintain a SECRET security clearance eligibility, with no access to classified information, is required. • Enroll in the Personnel Reliability Program (PRP), if required. Preferred Qualifications: • ACTIVE SECRET is preferred • Ph.D. preferred Pay and Benefits The salary range for this position is $80,000 - $90,000 annually. At Goldbelt, we value and reward our team's dedication and hard work. We provide a competitive base salary commensurate with your qualifications and experience. As an employee, you'll enjoy a comprehensive benefits package, including medical, dental, and vision insurance, a 401(k) plan with company matching, tax-deferred savings options, supplementary benefits, paid time off, and professional development opportunities.