Scientist I - Brain Health - Amyotrophic Lateral SclerosisWe are seeking a highly motivated Scientist to establish and optimize single cell long-read sequencing methods (e.g., PacBio HiFi/Kinnex or Oxford Nanopore), validate molecular and single cell assays, and execute experiments (e.g., in situ hybridization, immunostaining) to validate computational findings. Working closely with experts in neuropathology, spatial transcriptomics, and computational analysis, the Scientist will help advance high-resolution molecular atlases of the human nervous system in health and disease. The ideal candidate will have a strong foundation in molecular neuroscience, experience with single cell transcriptomic and/or epigenomic assays, and a working knowledge of ALS biology. Familiarity with long-read sequencing platforms and enthusiasm to establish novel molecular assays are also essential.
Essential Functions- Contribute to the design and execution of large-scale single cell genomics experiments
- Lead the development and testing of new long-read sequencing methods and protocols
- Optimize protocols for challenging samples, troubleshoot workflows for variable tissue quality, and evaluate tissue and sequencing library quality using standard metrics
- Conduct basic analysis of single cell and long-read genomics data, including benchmarking of new methods
- Present progress in group meetings and at scientific conferences, and contribute to peer-reviewed publications
- Assist in management of tissue acquisition and sample tracking
- Maintain meticulous experimental records to ensure reproducibility across tissue donors and adherence to best practices for tissue handling, biosafety, and data integrity
- Actively contribute to a collaborative environment focused on scientific rigor and methodological innovation
*Note: Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. This description reflects management's assignment of essential functions; it does not proscribe or restrict the tasks that may be assigned.*
Required Education and Experience- PhD degree in Neuroscience, Molecular Biology, Genomics, or related field, or equivalent combination of degree and experience
- Demonstrated experience conducting independent experimental research and interpreting complex biological data
- Broad knowledge of and experience with single cell genomics platforms
Preferred Education and Experience- Proficiency in independently conducting single cell genomics assays
- Experience establishing or optimizing long-read sequencing workflows (e.g., PacBio HiFi/Kinnex or Oxford Nanopore)
- Hands-on experience with human brain and/or spinal cord tissue, including dissection, nuclei isolation, and nucleic acid extraction
- Proven ability to troubleshoot complex molecular biology protocols and optimize workflows for variable sample quality
- Basic experience or familiarity with multicolor flow cytometry
- Basic familiarity with human neuroanatomy and willingness to expand anatomical knowledge
- Proficiency in tissue sectioning, histological stains, and/or immunofluorescence panel optimization
- Experience with Python and/or R for data analysis and visualization and an understanding of core single cell data analysis concepts
- Working knowledge of ALS pathology and molecular mechanisms of disease
- Excellent communication skills and comfort working in large, multidisciplinary teams to pursue collaborative research goals
Work Environment- Laboratory environment - possible chemical, biological, or other hazardous substances exposure
- Possible exposure to BioSafety Level 2 organisms, such as recombinant viruses
- This may include wearing personnel protective equipment (PPE)
- Working with dry ice or liquid nitrogen
- Sample handling of the following material, including but not limited to: human brain tissues, non-human primate brain tissues, human whole blood, PBMCs, plasma, nucleic acids, and cells
Physical Demands- Occasional lifting up to 30 pounds
- Fine motor movements in fingers/hands to operate computers and other office equipment, repetitive motion with lab equipment
- Stooping, bending, crouching
- Reaching or climbing ladders
Position Type/Expected Hours of Work- This role is currently working onsite and is expected to work onsite for the majority of working hours. The primary work location for this role is 615 Westlake Ave N., with the flexibility to work remotely on a limited basis.
Additional Comments- **Please note, this opportunity offersrelocation assistance**
- **Please note, this opportunity may offer work visa sponsorship**
Annualized Salary Range$86,150 - $106,650*
* Final salary depends on the required education for the role, experience, level of skills relevant to the role, and work location, where applicable.
BenefitsEmployees (and their families) are eligible to enroll in benefits per eligibility rules outlined in the Allen Institute's Benefits Guide. These benefits include medical, dental, vision, and basic life insurance. Employees are also eligible to enroll in the Allen Institute's 401k plan. Paid time off is also available as outlined in the Allen Institutes Benefits Guide. Details on the Allen Institute's benefits offering are located at the following link to the Benefits Guide: https://alleninstitute.org/careers/benefits.
