University of California San Diego

Laboratory Research Associate - 141572

Pharmaceuticals & Biotech
Less than 5 years of experience
Job Overview by Ladders

Qualifications

  • Advanced degree in Molecular/Cellular Biology or related field, or equivalent experience.
  • Proven experience with retroviral and lentiviral vector generation.
  • Skilled in molecular cloning and CRISPR techniques.
  • Experience in purifying red blood cell extracellular vesicles.
  • Proficient in flow cytometry, including operation of BD Facs Symphony and Cytoflex flow cytometers.
  • Demonstrated ability to mentor undergraduates and postdoctoral fellows.
  • Strong communication skills for effective documentation and collaboration.

Responsibilities

  • Independently perform and lead experiments on hematopoietic stem cell biology.
  • Isolate and culture mouse and human hematopoietic stem and progenitor cells.
  • Conduct in vivo transplantation of isolated cells.
  • Produce retroviral and lentiviral vectors for research purposes.
  • Implement CRISPR gene knockout and assess results using flow cytometry.
  • Analyze intracellular and cell surface proteins through RNA-sequencing.
  • Document research procedures accurately and follow established protocols.

Benefits

  • Comprehensive health and wellness programs.
  • Opportunities for professional development and continuing education.
  • Access to cutting-edge research facilities and resources.
  • Supportive work environment fostering diversity and inclusion.
Full Job Description
Payroll Title:
SRA 3 Department:
PEDIATRICS Hiring Pay Scale
$36.13 - $58.11 / Hour Worksite:
Moores Cancer Center Appointment Type:
Career Appointment Percent:
100% Union:
RX Contract Total Openings:
1 Work Schedule:
Days, 8-Hour Shifts, Monday-Friday

#141572 Laboratory Research Associate
Filing Deadline: Thu 10/15/2026
Apply Now

DESCRIPTION

The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 60 post- doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined, and are integral to the goals of the department.

The Department has undergone significant growth in recent years with a consolidated budget of approximately $106 million including sponsored projects expenditures of approximately $54 million and clinical revenue of over $ 52 million. This expansion of the Department's research and clinical portfolio is expected to continue in the next few years.

The Department's 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.

In 2001, physicians and leadership of the University of California, San Diego (UCSD), Rady Children's, and Children's Specialists of San Diego (CSSD) unified pediatric patient care, research, education and community service programs, creating a university-affiliated children's health system to serve the region. Over the past two decades, an extensive list of joint programs has developed, with many physicians and researchers playing a role at both institutions.

This affiliation has consolidated the clinical, teaching, research and public service programs of the UCSD School of Medicine's Department of Pediatrics with San Diego's only health care system dedicated to the health and well-being of children. In 2009, this partnership was furthered by the creation of Rady Children's Specialists of San Diego, a Medical Foundation, and is now recognized as having global leaders in research, technology, translational medicine, education and clinical excellence.

Under general supervision, the incumbent will independently perform and lead experiments related to hematopoietic stem cell and erythroid progenitor cell biology. The incumbent will isolate and perform ex vivo culture of both mouse and human hematopoietic stem and progenitor cells, and will also perform in vivo transplantation of isolated mouse and human hematopoietic stem and progenitor cells. The incumbent will produce retroviral and lentiviral vector and conduct CRISPR gene knockout followed by assessment with flow cytometry of both intracellular and cell surface proteins and RNA-sequencing. Performs other duties as assigned.

MINIMUM QUALIFICATIONS
  • Theoretical knowledge of Molecular/Cellular Biology or a related field, as typically attained with an advanced degree, or an equivalent combination of education and experience.
  • Experience with generation of retroviral vectors and lentiviral vectors.
  • Experience with molecular cloning.
  • Experience with CRISPR activation and CRISPR gene knockout.
  • Experience with purification of red blood cell extracellular vesicles.
  • Experience with advanced maintenance of cytoflex flow cytometer with plate loader.
  • Experience with independent operation of BD Facs Symphony cell sorter.
  • Experience with methanol cell permeation and intracellular flow cytometry and phospho flow cytometry.
  • Experience isolating mouse and human burst forming unit erythroid cells and colony forming unit erythroid cells.
  • Experience with tail vein and retroorbital injection of hematopoietic stem cells and hematopoietic progenitor cells.
  • Experience with creation and maintenance of stable 3T3 cell lines overexpressing c-Kit and CXCL12.
  • Experience with creation and maintenance of stable 3T3 cell lines overexpressing CRISPR-A.
  • Experience with creation and maintenance of stable 3T3 cell lines with CRISPR activation of Sca1.
  • Experience with flow cytometric analysis of primary erythroid differentiation culture.
  • Demonstrated ability to follow verbal and/or written instructions, including follow-up on previously begun procedures. Demonstrated accurate record data and enter into appropriate medium.
  • Demonstrated experience mentoring and overseeing undergraduates and postdoctoral fellows.
  • Experience with computer software such as Excel and Prism to analyze data, store results and compute raw data.
  • Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Demonstrated ability to read, comprehend and discuss research materials.
PREFERRED QUALIFICATIONS
  • Demonstrated experience managing UCSD Universal Scaled Storage cloud data storage.
  • Demonstrated experience establishing and maintaining cluster computing on the UCSD research computing cluster.
  • Demonstrated experience to adhere to established UCSD guidelines and policies.
SPECIAL CONDITIONS
  • Employment is subject to a criminal background check.
  • Occasional evenings and weekends may be required.
Pay Transparency Act

Annual Full Pay Range: $75,449 - $121,324 (will be prorated if the appointment percentage is less than 100%)

Hourly Equivalent: $36.13 - $58.11

Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for this position. The Annual Full Pay Range may be broader than what the University anticipates to pay for this position, based on internal equity, budget, and collective bargaining agreements (when applicable).

Apply Now

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community .

About University of California San Diego

The University of California San Diego (UC San Diego) is a public research university in San Diego, California. Established in 1960 near the pre-existing Scripps Institution of Oceanography, UC San Diego is the seventh-oldest of the 10 University of California campuses and offers over 200 undergraduate and graduate degree programs, enrolling approximately 38,325 students. UC San Diego is organized into six undergraduate residential colleges (Revelle, John Muir, Thurgood Marshall, Earl Warren, Eleanor Roosevelt, and Sixth College) and five academic divisions (Arts and Humanities, Biological Sciences, Jacobs School of Engineering, Physical Sciences, and Social Sciences). The university operates 19 organized research units (ORUs), including the Center for Energy Research, Qualcomm Institute (a branch of the California Institute for Telecommunications and Information Technology), and the San Diego Supercomputer Center.
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