One Rail Australia is an Australian rail freight operator company. Founded by Genesee & Wyoming Inc. in 1997 as Australian Southern Railroad, and later renamed Genesee & Wyoming Australia, it was renamed One Rail Australia in February 2020 after the company sold its remaining shareholding. In July 2022 it was purchased by Aurizon and the majority of One Rail's assets were transferred to that company; some remaining assets are set to be divested.