Department OverviewThe position is for a senior research associate responsible for experiments on MR1 antigen
presentation of mycobacteria in a BSL-2 setting. The position will require expertise in cell biology and cellular immunology techniques such as flow cytometry; expansion of human CD8+ T cells; sterile technique and cell culture of human cell lines, including BEAS-2B and THP-1; generation of primary human dendritic cells from peripheral blood mononuclear cells; working with primary human airway epithelial cells and primary human alveolar macrophages; RNA extraction, cDNA synthesis, and RT-qPCR; growing mycobacteria and performing accurate titers; using XRad320; and keeping accurate records of experiments in a digital lab notebook.
Function/Duties of Position- Plan, design, perform and analyze experiments for characterizing CD8+ T cell mediated killing of mycobacteria
- Sterile culture and expansion of human cell lines and primary human cells, including dendritic cells, macrophages, and airway epithelial cells
- Maintain accurate lab notebooks
- Other general lab duties as assigned
Required Qualifications- PhD in relevant field AND 1 year of relevant experience OR
- Master's Degree in relevant field AND 5 years of relevant experience OR
- Bachelor's Degree in relevant field AND 9 years of relevant experience.
Job Related Knowledge, Skills and Abilities (Competencies):- Design, setup and complete experiments using flow cytometry
- Analyze flow cytometry data using FlowJo
- Sterile culture technique
- growing and titering mycobacteria
- Experience performing RT-qPCR
Preferred Qualifications- 15+ years of relevant experience in basic science research
- Experience working with human CD8+ T cells and MR1 • Direct experience generating and working with dendritic cells
- >4 years of experience performing flow cytometry
- >4 years of experience performing interferon gamma release assays
- >1 year experience with XRad320
Additional DetailsMonday-Friday 4 hour workday in a research lab setting. Schedule may vary depending on project requirements and may infrequently involve working during evenings/weekends. Busy work environment with strict deadlines, interruptions, multiple demands and people interactions.
