Dec 27 at 1:00 PM
Neo-antigen discovery by an advanced method: http://ow.ly/ZlyC30hrA6O. T cell receptor (TCR) recognition of peptides on the human leukocyte antigen (HLA) determines T cell immune response. Therefore, identifying specific neoantigens for immunotherapy has attracted great attention both in academic laboratories and pharmaceutical companies. Now, Garcia and colleagues developed a novel method combining yeast display library screening and single-cell TCR characterization through sequencing, which was reported online in Cell. Compared to previous strategies, this approach is an unbiased and high-throughput manner due to the yeast display selection for enriching specific peptides. So, this technique will illuminate cellular details of T-cell mediated diseases. More importantly, it will be beneficial for the adoptive T cell therapies or vaccine design based on the identified neoantigens.