Job Description
About This RoleWe're seeking a Senior Research Associate who will be responsible for the culture of genetically modified primordial germ cells for the generation of chickens expressing human antibodies and other novel antigen-binding scaffolds. This position will interface between cell culture and molecular biology teams. This position represents a critical role in developing our next generation of innovative in vivo platforms for the discovery of novel therapeutics. A successful candidate would have strong technical skills and hands-on experience in primary cell culture and genetic modification.
The Animal Genetics group focuses on engineering chickens to produce human antibodies in various formats for different therapeutic applications. New genetically modified lines of chickens are validated in antibody discovery campaigns and the antibodies produced are characterized prior to the chickens being used in partner programs. The genetic modification of chickens requires culturing chicken germline cells and affecting genetic changes using modern methods of gene editing.
The ideal candidate has experience in stem cell culture, stem cell genetic modification, and molecular biology techniques.
This role reports to the Fellow in Animal Genetics.
This is an onsite position based out of our Emeryville headquarters. This is a full-time position, and the working hours are 8am-5pm PT.
What You'll Do - Propagate and genetically modify chicken primordial germ cells (PGCs), monitoring morphology and growth characteristics and correlating them with germline transmission
- Design and implement gene editing tools for precision genetic modification of PGCs
- Produce germline chimeras by microinjection of PGCs
- Establish re-derived PGC lines from transgenic animals and evaluate their germline competence
- Characterize PGC lines and transgenes by immunological methods, PCR and sequencing
- Evaluate novel antibody formats and gene editing strategies in immortalized B cells prior to PGC modificationby flow cytometry, PCR, RT-PCR and sequencing
- Collaborate with team members onimmunophenotyping of new bird linesand onroutine genotypingtasks
- Keep accurate and complete notebook entries with effective data management and visualization
- Maintain inventories of cell culture media and reagents, prioritizing replenishment of cell stocks as needed
- Present at OmniAb internal meetings on genetic modification work
What You Bring
- M.S. with 5+ years experience
- Experience with stem cell culture is required
- Experience with genetic modification of stem cells
- Experience in molecular biology
- Ability to prioritize tasks and complete projects on time
- Eagerness to learn new methods and apply knowledge to a steady stream of new projects with distinct goals
- Must demonstrate well-developed communication, record keeping, organizational skills, and the ability to work in a team environment
Nice To Have
- Experience with antibody discovery or characterization
- Experience with transgenic animals
Our total rewards package includes an annual base compensation, target bonus, equity, refresh grants, ESPP, comprehensive health plans, immediate 401k matching, PTO, sick leave, paid parental leave, commuter benefits, wellness stipend, education reimbursement and more!
The base compensation range for this position is $105,345 - $128,086 per YEAR. The range provided is an estimate. Actual compensation may vary based on skills, experience, education, location, and other factors permitted by law.
