We are seeking a Senior Molecular Technologist (Molecular Technologist III) to join our Laboratory Operations team to perform novel NGS based assays for the assessment of on- and off-target edits generated by gene editing technologies for cell and gene therapy applications. The ideal candidate will have extensive experience performing high-complexity molecular testing in a regulated laboratory environment, including automated NGS workflows, sequencing, quality control, equipment management, and technical troubleshooting. This individual will serve as a technical resource within Laboratory Operations, support the implementation and transfer of new assays, train junior personnel, and drive continuous improvement initiatives while remaining primarily hands-on at the bench.
Job Responsibilities:- Perform complex molecular biology and NGS workflows, including sample preparation, nucleic acid processing, PCR, library preparation, library purification, quality control, sequencing, and other downstream processing activities.
- Execute testing in accordance with approved Standard Operating Procedures, study-specific instructions, development protocols.
- Operate and maintain automated liquid handling platforms, NGS sequencers, and other molecular laboratory equipment, including pipettes, thermocyclers, centrifuges, fluorometers, and fragment analysis instruments.
- Lead or troubleshoot assay failures, equipment malfunctions, automation errors, and unexpected quality control results.
- Support assay, instrument, and automation validation activities, including equipment qualification, technology transfer, verification, and implementation.
- Manage multiple concurrent workflows while maintaining accuracy, documentation quality, and turnaround time commitments.
- Lead or substantially contribute to investigations of deviations, nonconformances, incidents, assay failures, and other quality events, including root cause analysis and corrective and preventive actions.
- Author, review, revise, and implement SOPs, work instructions, forms, training materials, validation documents, and other controlled quality records.
- Support laboratory inventory management, reagent qualification, vendor evaluation, material forecasting, and implementation of alternative supplies or vendors.
Requirements
- Bachelor's degree in clinical laboratory science, medical technology, molecular biology, biochemistry, genetics, chemical or biological sciences, or a related discipline.
- 5+ years of relevant molecular laboratory experience, including experience in a regulated, clinical, GLP, GMP, ISO or similarly controlled laboratory environment.
- Demonstrated experience with automated NGS workflows, laboratory documentation, and quality systems in a regulated environment.
- Demonstrated ability to independently troubleshoot complex molecular workflows and drive laboratory process improvements. Demonstrated ability to manage multiple laboratory activities while maintaining accuracy, quality, and turnaround-time commitments.
- Excellent written and verbal communication skills.
Preferred Qualifications- Experience with CRISPR gene editing, off-target analysis, or cell and gene therapy applications.
- Experience with hybrid capture, targeted enrichment, high-throughput sequencing, or complex multiplexed library preparation.
- Experience with amplicon-based sequencing, targeted amplicon panel workflows, or similar targeted NGS applications, including library preparation, amplification, pooling, quality control, and sequencing.
- Master's degree in molecular biology, genomics, bioinformatics, biotechnology, or a related scientific discipline or ASCP certification in molecular biology or another relevant laboratory certification.
Work Environment & Logistics- Full-time, exempt position
- 100% on-site in Waltham, Massachusetts
- Standard work schedule Monday through Friday
- No travel required
MaxCyte offers a base salary, annual bonus, and equity. MaxCyte also offers a comprehensive benefits package including health, dental, vision, life, and disability insurance and generous time off.
