AbbVie

Scientist l, Cell Line Development (based fully onsite)

AbbVie • $95K — $115K *
South San Francisco, CA 94080In-Person
Pharmaceuticals & Biotech
Less than 5 years of experience
Today
Be an Early Applicant
Job Overview by Ladders

Qualifications

  • Bachelor's Degree with 5+ years or Master's Degree with 2+ years in biologics or related field.
  • Hands-on experience in aseptic technique and mammalian cell culture, especially CHO cells.
  • Proficient in nucleic acid extraction and PCR analysis, including qPCR and/or ddPCR.
  • Experience in developing stably transfected CHO cell lines for monoclonal antibodies.
  • Hands-on with transposase/recombinase technologies in cell line development.
  • Knowledge in cryopreservation and management of CHO cell banks.
  • Familiar with quantifying recombinant protein titer using biolayer interferometry and screening assays.

Responsibilities

  • Design and conduct experiments for CHO cell line development and characterization.
  • Execute workflows for transfection, selection, screening, and scale-up of CHO cell lines.
  • Perform genetic characterization and support vector design for novel proteins.
  • Generate and maintain monoclonal CHO cell lines and associated cell banking records.
  • Assess clone productivity and stability through fed-batch culture studies.
  • Maintain and operate analytical and cell culture instrumentation.
  • Develop and optimize methods for reliable experimental data generation.
  • Document experimental processes accurately and train new team members.
  • Facilitate cross-functional collaboration to meet project timelines.
  • Ensure compliance with safety and regulatory standards.

Benefits

  • Comprehensive benefits package including paid time off and medical/dental/vision insurance.
  • 401(k) plan for eligible employees.
  • Participation in short-term incentive programs.
Full Job Description
Job Description

The Cell Line Development group in South San Francisco, CA is seeking a Scientist I to join a talented and collaborative team developing CHO cell lines for GMP applications. In this hands-on laboratory position, you will execute CHO cell line development and characterization studies in a well-supported, fast-moving environment that advances novel biotherapeutics into human clinical studies. The work spans molecular, cellular, and bioprocess development activities supporting the generation and characterization of optimized CHO cell lines for our biologics pipeline candidates.

This is a bench-focused position on a small team where each scientist owns their projects end to end and works directly with partners across process and analytical development. Our group runs both platform and non-platform molecules, so the work varies considerably from project to project.

Responsibilities
  • Experimental design and interpretation. Perform both routine and complex laboratory tasks competently and independently, generating reliable and consistent results. Independently design and conduct critical experiments that further project goals. Interpret results and draw conclusions from your own multistage experiments, note significant deviations, and then suggest, design, and pursue the follow-up experiments those results call for.
  • Cell line development execution. Execute CHO cell line development workflows for pipeline and innovation projects, including transfection, selection, high-throughput clone screening, and culture scale-up from multi-well plates through shake flasks and small-scale stirred tank vessels. Apply both random and targeted integration approaches, including transposase- and recombinase-mediated systems.
  • Molecular and genetic characterization. Extract DNA and RNA from CHO cell lines and cell banks and perform genetic characterization by PCR, qPCR/RT-qPCR, ddPCR, and sequencing-based methods. Support vector design, construction, and sequence confirmation for antibodies and novel protein formats.
  • Clone generation and cell banking. Perform single-cell cloning to establish monoclonal CHO cell lines. Cryopreserve research cell banks and maintain accurate bank inventory records.
  • Productivity and bioprocess assessment. Design and run fed-batch and parallel culture studies, on both manual and automated platforms, to evaluate clone productivity, growth, and stability.
  • Instrumentation. Operate and maintain the group's analytical and cell culture instrumentation, including automated cell counters (Vi-CELL) and label-free binding platforms (Octet).
  • Method development and assay support. Investigate, identify, develop, and optimize new methods and techniques to generate reliable and reproducible data in a timely manner, reading and adapting the scientific literature to accomplish assignments and demonstrating broad and versatile technical expertise within the discipline.
  • Contribute to the qualification and troubleshooting of the assays that underpin clone selection, implement and where possible innovate new experimental protocols, and identify opportunities to improve throughput, data quality, and overall workflow efficiency.
  • Documentation and knowledge transfer. Maintain meticulous, contemporaneous records of experimental design, data, and analysis in electronic lab notebooks (ELNs), in compliance with AbbVie's standards for data integrity and good documentation practices. Author and revise protocols, and help train new team members on cell culture and characterization techniques.
  • Cross-functional collaboration and communication. Present scientific updates at team and cross-functional meetings and partner closely with colleagues in process development and analytical groups to drive projects forward and meet aggressive timelines.
  • Compliance and safety. Understand and adhere to corporate standards regarding code of conduct, safety, the appropriate handling of materials, and GxP compliance.


Qualifications
  • Bachelor's Degree or equivalent education with typically 5+ years of experience, or Master's Degree or equivalent education with typically 2+ years of experience in biologics discovery or development, or a closely related discipline.
  • Hands-on experience in aseptic technique and mammalian cell culture, with a primary focus on CHO cells, including the ability to manage multiple cell lines and projects in parallel.
  • Proficiency in nucleic acid extraction from mammalian cells and PCR-based analysis, including qPCR and/or ddPCR.
  • Hands-on experience developing stably transfected CHO cell lines to produce monoclonal antibodies or antibody-like molecules, including transfection, selection, minipool isolation, clone isolation and screening, and scale-up.
  • Hands-on experience with transposase/recombinase technologies in CHO cell line development.
  • Practical experience cryopreserving CHO cells and generating research cell banks, including assessment of post-thaw viability and recovery, and accurate inventory record-keeping.
  • Experience quantifying recombinant protein titer by biolayer interferometry (e.g., Octet) or an equivalent titer method.
  • Experience running plate-based screening assays to rank large numbers of clones in 96-well and 24-deep-well plate formats, including plate mapping, scheduled sampling, and review of the resulting data for outliers and plate effects.
  • Experience evaluating clone productivity, growth, and stability in batch or fed-batch culture, including scheduled sampling, data analysis, and ranking of candidate clones on manual and/or automated platforms.
  • Demonstrated ability to independently design, execute, interpret, and troubleshoot experiments that answer defined scientific questions and advance project goals, including sound analysis of quantitative data, the judgment to recognize significant deviations, and the initiative to design the follow-up experiments they call for.
  • Strong understanding of mammalian cell biology and molecular biology concepts, with the ability to draw on relevant scientific literature and apply theoretical frameworks to solving problems within the discipline in a timely manner.
  • Excellent attention to detail, with a strong commitment to maintaining detailed, daily records in an electronic lab notebook.
  • Strong written and verbal communication skills, including the ability to present data clearly to colleagues and cross-functional partners.


Additional Information

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:
  • The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.
  • We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.
  • This job is eligible to participate in our short-term incentive programs.

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employee remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company's sole and absolute discretion, consistent with applicable law.

About AbbVie

AbbVie develops pharmaceuticals and medical devices. They provide products and services to therapeutic areas including immunology, oncology, neuroscience, eye care, virology, women's health, and gastroenterology.

AbbVie Careers

Joining AbbVie means becoming part of a global team dedicated to making a remarkable impact on patients' lives. At AbbVie, our employees are united in the pursuit of groundbreaking innovation and are committed to transforming the future of healthcare with leading-edge science.

Work You’ll Do

At AbbVie, you’ll collaborate with some of the brightest minds in the industry to solve challenging problems that have a high impact on society. Our culture fosters growth and embraces leadership and diversity training, ensuring that every team member can thrive.

Explore Job Opportunities

AbbVie offers a wide range of job opportunities and career paths, providing a platform where professionals can propel their careers forward. From research and development to marketing and sales, the potential to make a significant impact is limitless.

Internship Programs

Kickstart your career with an AbbVie internship. Our programs provide invaluable industry experience and a chance to develop essential skills in a real-world setting. Interns at AbbVie are considered integral members of the team and are given tasks that are both challenging and rewarding.

Professional Growth and Development

We believe in nurturing our team's professional growth through comprehensive training programs, leadership development opportunities, and continuous learning. Our commitment to your career growth is reflected in our robust offerings that enhance your skills and knowledge.

Benefits and Culture

AbbVie is dedicated to supporting our employees' well-being both inside and outside of work. Our benefits package includes health, financial, and social benefits that are designed to support the diverse needs of our employees. Our inclusive culture encourages collaboration and innovation, fostering a workplace where all can excel.

Hiring Process

Our hiring process is designed to ensure a match that will be beneficial both for the company and for your career aspirations. From resume submission to interview, each step is an opportunity to showcase your skills and fit with the AbbVie team.

Networking and Career Advancement

At AbbVie, networking doesn’t just enhance your career; it propels it. We encourage our employees to engage internally and externally to build relationships that foster personal and professional growth.

Join Our Team

Search open positions that match your skills and interests. We are looking for passionate, curious, and innovative team players who are ready to make a difference.

Stay Connected

Keep up to date with career tips, insider perspectives, and industry-leading insights you can put to use today—all from the people who work here.

Job Alert Emails

Personalize your subscription to receive job alerts, latest news, and insider tips tailored to your preferences. Discover the exciting and rewarding opportunities that await at AbbVie.

Explore AbbVie Jobs

Whether you’re seeking to advance your career in a dynamic and empowering environment, or looking for a place where you can innovate, lead, and contribute to something bigger, AbbVie is the place for you. Join us in our mission to discover and deliver innovative medicines that solve serious health issues today and address the medical challenges of tomorrow.
Learn more about AbbVie
Size
50,000 employees
Market Cap
$288.5 billion
Industry
Pharmaceuticals & Biotech
Net Income
$4.6 billion
Founded
2013
5 Year Trend
+17%
Revenue
$45.8 billion
NASDAQ
ABBV
* Ladders Estimates

Similar Jobs

Ad banner background

Get Ready For Your
Next Interview

More Jobs at AbbVie

More Pharmaceuticals & Biotech Jobs

Find similar Scientist l, Cell Line Development (based fully onsite) jobs:

NationwideSouth San Francisco, CA