This position is full-time. Hours are generally normal business hours but may vary due to the timing of experiments and other research activity deadlines.
Department Name/Job Location
This position is in the Department of Internal Medicine – Division of Cardiology. This position is for the Medical School Campus.
The interest of this academic laboratory (PI: Babak Razani) is understanding the mechanisms of atherosclerosis, diabetes, obesity, and fatty liver disease with special emphasis on the roles of macrophages. We and others have shown that perturbations in cellular processes involving autophagy, lysosomes, and inflammasomes play a critical role in macrophage dysfunction and subsequent cardiometabolic disease (reviewed in Evans, et al Science Signaling 2017). Some of our relevant primary papers include (Sergin, et al Science Signaling 2016 and Sergin, et al Nature Communications 2017). The primary focus of the lab will be to understand the mechanisms by which these cellular processes affect metabolism, determine if their manipulation ameliorates metabolic disease, and translate our findings to develop novel therapeutics in human coronary artery disease, obesity, and insulin resistance.
These questions are being studied using a combination of molecular and cell biology approaches in mouse primary cells/cell-lines, mouse models, human monocytes/monocyte-derived macrophages, and induced pluripotent stem cell (iPSC)-derived macrophages.
Candidates with extensive prior experience in cell, molecular biology or related fields with a Ph.D. or M.D. degree are encouraged to apply.
- Mammalian cell culture experience.
- Basic microbiology skills.
- Experience with microscopy, especially confocal/fluorescence microscopy.
- Proficiency in basic molecular biology techniques including DNA/RNA/protein isolation and purification, PCR, cloning, reverse transcription PCR, immunoblot, and ELISAs.
- Experience with immunocyto- and histo-chemistry and frozen/paraffin tissue.
- Experience with flow cytometry and immunology.
- Ability to handle mice, perform injections, and harvest tissue.
Base pay for this position is commensurate with experience.