The purpose of this position is to provide advanced, doctorate level scientific research for the Miano laboratory at Cardiovascular Research Institute (CVRI). Under general direction and with considerable latitude for exercising independent scientific judgment, this position provides direct research contributions to the laboratory by directing small, complex research projects and/or major aspects of larger research projects. Provides guidance and/or directly supervises research technicians, graduate students, PhD and Post-Doctoral laboratory members. May work as an independent researcher in a scientifically unique phase of research as part of a larger, complex project. Importantly, the candidate must be able to work well with others, and be a proficient communicator/ instructor. The position requires an understanding of the scientific process that facilitates grant and manuscript generation, and a familiarity with the workings of the regulatory bodies of the University to allow for a productive lab environment.
- Designs and implements complex research projects and unique sub-projects related to long noncoding RNAs and CRISPR-Cas9 genome editing in mice and in cells. Contributes to the final writing of scientific research publications and thereby meeting the requirements for co-authorship. Writes protocol and operating procedures for the conduct of scientific research using Photoshop, Statistical software, PowerPoint, and other appropriate software which include: purposes of study, design of experiments, list(s) of materials and methods used and the schedule of activities.
- Working closely with two post-doctoral fellows, will carry out studies of lncRNAs and an array of protein-coding genes undergoing CRISPR editing in mice using a variety of methods in molecular biology including, but not limited to, Western blotting, Northern blotting, qRT-PCR, 5’ and 3’ RACE, luciferase assay, pulldown and Co-IPs.
- Analyzes and evaluates scientific results of complex and novel experimental data and provides interpretation of results within scope of the study. Examines and assesses the importance of scientific findings in relation to the general areas of molecular biology, biochemistry, cell biology. Applies significant scientific knowledge and expertise in the specific scientific scope of extracellular matrix remodeling using multiple approaches.
- Develops and implements operating procedures for the laboratory and independently operates complex scientific equipment. Develops and modifies test methods; selects procedures for data collection and data handling. Develops, utilizes or adapts computer programs for statistical treatment and evaluation of scientific data.
- Applies advanced scientific knowledge and expertise for the purpose of guiding and advising internal colleagues on scientific technical problems within the scope of research and making recommendations for corrections, especially as they relate to grant submissions.
- Provides scientific guidance to staff/trainees. Advises on technical problems and assists staff/trainees with special research projects.
Doctoral degree with a major course work in the field of assignment and a minimum of 2-5 years of relevant postdoctoral training. Preferred: Ph.D. and/or MD with demonstrable expertise in molecular biology with a minimum of 2-5 years of relevant postdoctoral training.