Scientist III, Cell Biology at Thermo Fisher Scientific in Carlsbad, CA

Job Description

Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue exceeding $25 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, improving patient diagnostics and therapies or increasing productivity in their laboratories, we are here to support them.

How will you make an impact?

Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing.

What will you do?

The successful individual will directly interact with senior R&D scientists and project management to execute project work plans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.

This role will involve the following tasks:

• Mammalian cell culture
• DNA delivery and mammalian cell transfection
• Generation of Lentivirus
• Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
• Flow cytometric analysis
• Molecular biology
• Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
• This individual will also be expected to support and participate in R&D and project management meetings.

Knowledge, Skills & Experience

• Proven experience in cell biology
• mammalian cell culture
• Lipid-based cell transfection and electroporation
• Cell engineering (CRISPR or TALEN)
• Stable cell line generation
• Cell-based assays, such as FACS, immunocytochemistry, ELISA, and Western Blotting
• Experience in virology
• Deliver packaging plasmids into cells
• Generation of Lentivirus
• Measurement of viral titers
• PCR, qPCR assays
• Experience in molecular biology
• DNA cloning and sequencing
• DNA, RNA preparation
• PCR, qPCR assays
• Ability to:
• Observe deviations from the expected outcomes and trouble-shoot
• Succeed with a moderate level of supervision and guidance
• Participate in a strong teamwork orientation with demonstrable influence/interpersonal skills
• Maintain a detailed lab notebook and present your findings in a group setting

How will you get here?

Education/Experience:

• A master’s degree with 5+ years of experience in cell biology.
• PhD with 2+ years of experience in cell biology