Scientist I - Protein Purification

Confidential Company  •  Carlsbad, CA

Less than 5 years experience  • 

Salary depends on experience
Posted on 06/09/18 by Chitrank Rastogi
Confidential Company
Carlsbad, CA
Less than 5 years experience
Salary depends on experience
Posted on 06/09/18 Chitrank Rastogi

*         Responsible for manufacturing product(s) in a lab setting following standard operating procedures.

*         Will utilize basic laboratory techniques, calculations, materials measurement, operation of laboratory equipment and record keeping necessary to manufacture products.

*         Will perform column purification/ chromatography, buffer preparation, SDS-Page Analysis, Western Blotting, and other protein characterization techniques.

*         Responsible for Batch Data Records, notebooks, customer reports and work order documents.

*         Utilize specialized technical knowledge and skills to make detailed observations and analyze data to ensure within operating parameters.

*         Advise manager of factors that may affect quality of product.

*         Suggest options/ideas for corrective actions.

*         Maintain equipment, product inventories and related records as assigned.

*         Note variances on manufacturing documents; audit and evaluate process documents and propose revisions.

*         Complete, distribute and file records as required in accordance with Document Control Practices.

*         Identify potential issues in the production process; report findings to team management and members for resolution or participate in troubleshooting.

*         Write technical documents which may include standard operating procedures and batch records.

*         May lead process improvement projects or product transfer projects

*         May perform other related duties as required and/or assigned

Minimum Qualifications (must have):

*         Must have a bachelor's degree in Biology, Chemistry, Biochemistry, or other related discipline.

*         Masters degree is a plus.

*         Requires a minimum of 3 years relevant experience demonstrating laboratory techniques in a manufacturing environment or research laboratory.

*         Demonstrated proficiency in the use of scientific laboratory techniques, equipment and materials required.

*         Experience in the use of basic laboratory techniques, including buffer preparation, column purification/ chromatography, SDS-Page Analysis, Western Blotting, and other protein characterization techniques are beneficial.

*         Familiar with GE AKTA FPLC protein chromatography equipment and Unicorn software.

*         Experience with different modes of chromatography such as: affinity, ion-exchange, size exclusion, hydrophobic interaction.

*         Assessment of purified kinases through radioactive activity, fluorometric plate based assays, and TR-FRET.

*         Lifting up to 20 lbs will be required on a frequent basis.

*         May be required to stand for long periods of time while performing duties.

*         Must be flexible to work varying schedules and hours as needed.

*         Must be able to work safely with chemicals and hazardous materials.

Preferred Qualifications (nice to have):

*         Consistent, dependable and accurate in carrying out responsibilities

*         Displays positive attitude toward job and others

*         Follows and provides direction accurately and efficiently

*         Excellent documentation and communication skills

*         Demonstrated ability to carry out scientific procedures and make recommendations for improvement required.

*         Good problem solving skills necessary.

*         Working knowledge of manufacturing related computer software preferred.

*          Intermediate Excel skills.

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