Scientist, Cell Line Generation

Shire plc   •  

Cambridge, MA

11 - 15 years

Posted 246 days ago

This job is no longer available.


This position is within Shire’s Cell Line Generation (CLG) group. Its key role is to conduct mammalian cell culture for the generation, characterization, and maintenance of cell lines supporting clinical and commercial drug product manufacturing. The incumbent will develop, maintain and operate high throughput automation equipment used to support CLD.

The responsibilities of this role include independent planning, designing and execution of scientific experiments, interpreting and presenting scientific data. This candidate will have project coordinator responsibilities for Cell Line Generation (internal or external) and will actively provide scientific expertise to Discovery and Development teams as needed. Other duties can include significant molecular biology, transfection, drug selection, single cell isolation, cell counting, cryopreservation, process and metabolite analysis.

Significant preference given to candidates with experience operating and/or developing assays related to Berkeley Lights technology. Candidate must have experience with automated 250mL through 10L bioreactors as well as familiarity with upstream process development.

Must be flexible with assignments and adaptable to the changing needs of projects. This person must be self-motivating and hard-working. Attention to detail is required.

Resonsibilities (% of time):


Research Activities including experimental planning and direct laboratory activities, relevant and necessary literature review.


Communication with supervisor regarding experimental designs and results. Communication with members of Discovery Biology and Discovery Therapeutics, Process Development and Manufacturing Departments. Helping of team members to succeed in scientific experiments. Consultation with outside experts and collaborators.


Report writing and record keeping.


          A Life Sciences with emphasis on Molecular Biology or Cell Biology, and with five years of relevant working experiences. A Masters Degree with 7years of industrialexperience, or a Bachelors degree with 10 years of experience.

Key Skills and Competencies:

Knowledge in Molecular cloning and cell line development, cell expression, purification and protein analytics is required. Experience with development of fluorescent assays and FACS based technology. Capability in designing expression constructs for mammalian, baculo-virus or bacteria expression system, performing transfection and transformation to overexpress recombinant proteins, evaluating the productivity of cell lines by biochemical methodology such as small scale purification, ELISA, enzyme activity assay and Western blot analysis, are required. Implementation of new technologies to suit the changing needs of the Discovery Projects is necessary.

Job role to include operation of small scale bioreactors and tech transfer to upstream process development and cell bank manufacturing groups both internal and external.

Req ID: R0018364