Research Scientist III

Children Hospital seattle   •  

Seattle, WA

5 - 7 years

Posted 234 days ago

This job is no longer available.


The James ( and Rawlings labs at Seattle Children’s Research Institute are hiring motivated senior scientists to join a rapidly expanding research program focused on B cell engineering (PMID 29273498). The ultimate goal of the B cell engineering group is to develop novel cell therapies that provide sustained production of therapeutic proteins from gene-edited, adoptively transferred, autologous plasma B cells. For this position, we are seeking a highly motivated and experienced immunologist/molecular biologist to lead projects in the primary B cell engineering and differentiation sub-group. The applicant will build upon existing engineering approaches to expand gene-edited human B cells in culture, differentiate gene-edited B cells into long-lived plasma B cells and further enrich for therapeutic protein secreting cells. The applicant will collaborate with the principal investigators to manage the project and personnel and to develop the mid-term experimental plans. Additionally, the applicant will be responsible for communications with external collaborators and will analyze and interpret data and contribute to grants, patents and manuscripts.  


Required Education/Experience: 
- Master’s Degree or higher in molecular biology, immunology, bioenginnering, virology, or a related field; may require a PhD for certain lab specialties 
- Minimum of three (3) years experience in immunology, preferably with a background in B cell biology, and/or molecular biology, preferably with a background in genome engineering and/or generation and use of viral vectors
- Hands-on expertise with flow cytometry, ELISA, ELISpot, and/or intracellular staining

- Expertise in protocol development and troubleshooting

- Recent evidence of publication
- Experience with laboratory coordination, working with research staff and training technicians
- PhD in molecular biology, immunology, bioenginnering, virology, or a related field
- Extensive expertise in the culture of primary human cells

- Strong presentation and/or writing skills

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