Not Specified years
Posted 50 days ago
Post-doctoral positions are available to investigate stem cell approaches to treat neurogenetic diseases in brain and retina. The position is working in the laboratory of Dr. John Wolfe, a Principal Investigator within the Neurology Research Division of the Research Institute. The development of somatic cell reprogramming into iPSCs and then into NSCs raises the potential of creating patient-specific cell therapies. Two approaches are being studied under NIH funded projects.
1) Retinal Photoreceptor Replacement in Blind Animals: Many retinal degeneration diseases will require restoration of neuroretinal cells. As part of the NIH National Eye Institute's Audacious Goals Initiative, we are developing new iPSC-derived retinal progenitors for transplantation into large animal models of human blinding diseases. The project involves developing retinal organoids (retinas-in-a-dish) to differentiate into photoreceptor precursor cells. The studies involve developmental analyses, exome and phenotypic comparisons between animal model and human organoids, and evaluating engraftment and functioning in diseased retinas.
2) Neural Stem Cell Migration within the Brain: The natural ability of neural stem cells (NSCs) to migrate within the brain, under certain circumstances, has long made them a candidate for treatment of CNS diseases. However, significant barriers to effective delivery continue to impede progress. Most neurological diseases have globally distributed lesions within the CNS due to the nature of the defect and treatment requires disseminated distribution of the donor cells. However, donor cells currently engraft at sub-therapeutic levels. We are investigating factors that affect post-injection survival and migration, including ex vivo culture conditions and genetic manipulations. The long-term goal is to develop NSC transplants in the mouse brain to a level where they can be studied in large brain animal models for translation to the human brain, which is ~3,000 times larger than the mouse brain.
Postdocs will also be expected to acquire technical, lab management, and manuscript/grant writing skills; and participate in seminars, lectures, poster sessions and presentations at national meetings. Postdoctoral fellows also may be required to supervise junior lab members, develop new methods and protocols for research, and assist with the development of other research projects in the lab. The Postdoc experience will serve to extend, refine and enhance skills necessary for professional and career development, and will enable the individual to broaden his/her scientific background by acquiring new research capabilities. It is expected that this individual will conduct independent scholarly research, and will contribute directly to the overall research goals of the project and the research group. Postdocs will be expected to participate in project planning, recording and interpretation/evaluation of data, and communication of results.
Required Education and Experience
Req ID: 23553